Abstract: Objective The present study was conducted to monitor the related serological prevalence in men who have sex with men (MSMs) in Wuhan City in order to find out the situation of sexually transmitted diseases (STDs) and HIV infection in the population and provide a scientific basis for effective measures of intervention，prevention and control of the diseases．Methods 145 MSMs received questionnaire survey and their venous blood was sampled to detect the related antigens or antibodies of HIV，hepatitis B virus，hepatitis C virus，treponema pallidum，and chlamydia trachomatis of urogenital tract．Results A total of 145 MSMs were tested．HIV-Ab，HBsAg and HCV-Ab were positive in 2 (1.38％)，16 (11.03％)，and 1(0.69％) cases respectively．Treponema pallidum particle agglutination assay (TPPA) was positive in 44 (30.34％) cases，among which rapid plasma reagin assay (RPR) was positive in 22 (15.17％) cases．IgG of chlamydia trachomatis of urogenital tract (CT) was positive in 16 (11.03％) cases．Conclusion The infection rate of STDs was quite high among MSMs．Therefore，it is urgent to conduct effective measures of prevention and control of STDs and AIDS in the population．