Abstract: Objective The present research was designed to isolate measles virus from patients with measles to get more isolated virus swains for identification of genotypes of the prevalent vimlent strains of measles viruses in China Methods Throat swab samples of selected 109 suspected patients with measles admitted to our hospital were collected from May to August of 2005，and the marmoset B cell line transformed by Epstein-Burr virus (B95a) was inoculated．Results Forty-six strains of measles viruses were isolated with a positive isolation rate of 42.20％．Conclusion Wild types of measles virus were isolated by the Second Hospital in cooperation with Jiangsu Provincial CDC．Large number of samples was collected with a high positive rate It is concluded that the good results are closely related to good skills of technical personnel,good timing of collection and timely cold chain storage and transportation。