Abstract: Objective The study was conducted to understand the infection and drug resistance of ureaplasma ureaplycium (UU) and mycoplasma hominis (MH) among patients of nongonococcal urethritis (NGU)in Yuyao City，as well as the distribution of infected people．Methods An integrated kit for isolation，identification，counting and drug resistance test of mycoplasma was used for testing the positive rate of culture and the drug resistance of the mycoplasma．Results Among the 896 specimens of patients，495 were found positive UU，and 23 were found positive MH．The dectection rate of UU for the female was 65.00％．While for the male was 46.64％．The infected people were mainly young and middle-aged people from 22 To 39 years old．The infection rate of MH was relative low，but with a broad spectrum of drug resistance，and the drug resistance of UU was acceptable，but relevant antibiotics was still to be selected reasonably．Conclusion For nongonococcal urethritis (NGU)，UU is one of the most common pathogenic microorganisms，and the detection and drug sensitivity test of UU may provide effective instruction and support for reasonable clinical application of antibiotics．