Abstract: Objective This study was designed to research the pathogen and analysis the outbreak and sporadic epidemic of adenovirus occurred in Hubei,Jiangxi,Jiangsu Province and Beijing Municipality in order to learn the infection status of adenovirus in China．Methods Pathogenic studies of 16 strains of adenovirus isolated were implemented，in which virus isolation，RNA extraction，PCR and sequencing were made．On the basis of epidemiological data，the epidemic charicteristics of adenovirus was summarized and analyized．Results The results showed that all of the 16 isolates were adenovirus 3 and no significance difference was observed among those strains．Conclusion Adenovirus type 3 is the main type that caused school ages and young children adenovirus infection and there was no significant gene mutation among these diflferent provincial isolates．