Abstract: Objective The present study was designed to find out the sero - epidemiological characteristics of HAV and HEV in natural population in Huzhou city for the development of preventive and control strategies. Methods A random sampling method was used to select subjectsfrom the plain rural region of rivers and lakes, the mountain rural area and the towns in the city ofHuzhou, with a total of 1666 healthy individuals included and clustered into 3 groups, in whom casestudy and serological detection of anti - HAV - IgG and anti - HEV - IgG were conducted on an epidemiological basis. Results The standardized infectious rates of HAV and HEV in the natural population in the city of Huzhou were 55.74% and 38.88% ,respectively, which were positively correlated with age. The infectious rate of HAV in towns was higher than that in the plain rural regionof rivers and lakes, while the rate of HEV in towns and the region was higher than that in mountain rural area. Both the infectious rate of HAV and HEV had positive correlations with ages. The co-infection rate of HAV and HEV was 32.23% with a statistical association between them. HEV and co-infection of HAV and HEV presented a trend of familial aggregation. Conclusion It is advisable todetect HEV at the same time of HAV diagnostic test. Prevention and control of the epidemics shouldbe conducted based on their epidemiological characteristics.