Abstract: Objective The present study was conducted to explore the current situation of viral hepatitis B prevalence in Quzhou. Methods The incidence and inoculation of hepatitis B in 2005 was taken into the analysis. 717 human sera were collected into the enzyme-linked immunosorbent assay (ELISA) for the hepatitis B virus surface antigen (HBsAg) and hepatitis B virus surface antibody (anti-HBsAg) detection. Results The incidence rate of hepatitis B in Quzhou in 2005 was 43.14/lakh, while the inoculation rate of hepatitis B was above 95% among children. The HBsAg positive rate was averagely 3.21% among the crowd, and anti-HBsAg positive rate 43.38%, averagely. Conclusion Inoculations for older children and adults should be strengthened. It is required that knowledge of hepatitis B be publicized in rural areas to raise the awareness of self-protection.