Abstract: Objective This study was conducted to determine the characteristics of antibiotics resistance and virulence gene of Vibrio cholera non-O1 which caused food poisoning through molecular biological methods. Method Antibiotics resistance analysis on Vibrio cholera non-O1 isolates was performed with drug sensitivity test. The virulence gene Ctx, Zot and Ace were detected by PCR. The molecular types were then classified by pulsed field gel electrophoresis (PFGE) and homology analysis was conducted. Results A total of 7 Vibrio cholera non-O1 strains were isolated from 8 patients and 8 food samples. Virulence gene analysis showed that Vibrio cholera non-O1 isolates carried none of 3 virulence genes(Ctx、Zot、Ace). All the isolates were positive to hemolysin. Classification of molecular types with PFGE and Dienes Test showed the isolates from patients and those from leaving food samples were homologous. The antibiotics resistance analysis showed that the isolates were sensitive to almost all the tested antibiotics except ampicillin and SMZco(resistance rates were 100%). Conclusions This experiment confirmed that the food poisoning occurred from eating were confused chicken claw. Retrospective epidemiology revealed homologous strains from the virulence genes of Vibrio Cholerae non-O1 in remaining cold dish and seafood, resistance and PFGE analysis.