Abstract: The study was conducted to investigate the distribution and drug resistance of Pseudomonas aeruginosa in Intensive Care Unit (ICU) patients along with the preventive measures. Relevant clinical data of ICU patients in the Traditional Chinese and Western Medicine Hospital in Wenzhou City, Zhejiang province from Jun. 2006 to Apr. 2007 were used for analysis. As a result, a total of 960 strains of pathogens were isolated among the 2190 infected individuals, which comprised 88 strains of gram-positive bacteria (9.2%), 746 strains of gram-negative bacteria (77.7%) and 126 strains of fungi (13.1%). The number of strains of Pseudomonas aeruginosa reached 296 (30.8%). Cefoperazone / sulbactam still maintained the highest antibacterial activity, whereas the resistance to imipenem and Tazocin increased significantly. Surveillance on drug resistance and properly guided administration of preventive medicine to reduce the selection of drug resistant strains are needed given the presence of strongly resistant ICU Pseudomonas aeruginosa. Targeted use of antibiotics is an effective infection control measures.