商晓春, 潘军航, 帅慧群, 赵雪琴. 一起O3:K6型副溶血性弧菌引起的食物中毒病原学检测[J]. 疾病监测, 2008, 23(4): 215-217. DOI: 10.3784/j.issn.1003-9961.2008.4.215
引用本文: 商晓春, 潘军航, 帅慧群, 赵雪琴. 一起O3:K6型副溶血性弧菌引起的食物中毒病原学检测[J]. 疾病监测, 2008, 23(4): 215-217. DOI: 10.3784/j.issn.1003-9961.2008.4.215
SHANG Xiao-chun, PAN Jun-hang, SHUAI hui-qun, ZHAo Xue-qin . Deletion of an etiological agent of food poisoning:Vibrio parahaemolyticus Serovar 03:K6[J]. Disease Surveillance, 2008, 23(4): 215-217. DOI: 10.3784/j.issn.1003-9961.2008.4.215
Citation: SHANG Xiao-chun, PAN Jun-hang, SHUAI hui-qun, ZHAo Xue-qin . Deletion of an etiological agent of food poisoning:Vibrio parahaemolyticus Serovar 03:K6[J]. Disease Surveillance, 2008, 23(4): 215-217. DOI: 10.3784/j.issn.1003-9961.2008.4.215

一起O3:K6型副溶血性弧菌引起的食物中毒病原学检测

Deletion of an etiological agent of food poisoning:Vibrio parahaemolyticus Serovar 03:K6

  • 摘要: 目的 检测食物中毒患者粪便或肛拭子、剩余食物、调味品、食物加工环节涂抹物分离的病原菌的生物学特性,确定食物中毒的病原.方法 参照GB/T4789--2003方法,对检出的副溶血性弧菌(vP)做血清分型、耐药性、种属特异基因和毒力基因tdh(耐热溶血素)和砌(耐热相关溶血素)检测.结果 从153份粪便或肛拭子样品中检出74株VP,并从调味品和加工环节涂抹物及垃圾桶内的剩余食物(猪头肉)中各检出1株VP,血清型均为03:K6型,神奈川溶血阳性(KP),77株VP聚合酶链反应(PCR)扩增都出现相同VP种属特异基因(320 bp)条带,77株副VP毒力基因复合PCR结果,tdh(434 bp)阳性,trh(250 bp)阴性.结论 本次食物中毒是由具较强毒力的03:K6型VP污染引起的.

     

    Abstract: Objective The study was conducted to identify the etiological agent that induced food poisoning by analyzing the victims' stool samples and/or anal swabs,food remains,condiments,and additives and spreads used during food processing to determine its biological characteristics.Methods The isolated strains of Vibrio parahaemolyticus were subject to serotyping,drug resistance test,species-specific gene tdh(Thermostable Direct Hemolysin) and virulence gene trh(Thermostable Direct Hemolysin-Related Hemolysin) detection per GB/T4789-2006.Results A total of 74 strains of VP were isolated from 153 stool samples and/or anal swabs,and other three were found one each from condiments,food-processing spreads and food remains in trash bags.The serotyping suggested that all strains of VP were serovar O3∶K6,Kanagawa positive.Amplification of the species-specific gene from the 77 strains resulted in an identical 320 bp band.The virulence gene multi-PCR test of them suggested tdh(434 bp) positive and trh(250 bp) negative.Conclusion Vibrio parahaemolyticus Serovar O3∶K6 was the cause of food poisoning;its strong virulence was confirmed.

     

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