Abstract: Objective　The study was conducted evaluate the on-the-spot performance of Colloidal Gold-Immunochromatographic Assay(GICA) in various epidemic areas of brucellosis. Methods Parallel comparison across GICA, dot immunogold filtration assay(DIGFA) and serum agglutination test (SAT) was made in different epidemic areas of brucellosis to evaluate the performance of the three methods in detecting human anti-brucella antibodies. Results　No statistical difference was found in the positive rates of human anti-brucella antibodies in epidemic areas, surveillance areas and non-epidemic areas detected by the three methods (P0.05). The positive coincidence was 94.2% between GICA and SAT when they were applied in epidemic areas, and the negative coincidence was 99.6% when applied in non-epidemic areas. The positive rates of human anti-brucella antibodies identified by GICA were statistically distinguishable across different epidemic areas (P0.01). Conclusion　Highly sensitive to potential patients, GICA can be used as a surveillance indicator of the extent to which the epidemic has prevailed. Its wide application is to be expected in different epidemic areas of burcellosis, which will contribute to the prevention and management of this disease.