Abstract: Objective The study was conducted to investigate the bacteria spectrum and the drug resistance in urinary tract infections (UTI) based on the surveillance data of UTI patients that visited the Peoples Hospital in Henan between 2003 and 2007, so as to provide evidence for the prevention and control of UTIs. Methods　Bacterial identification and drug resistance tests were performed for 1,959 strains of bacteria isolated from the urine culture of hospitalized and outpatients in the Peoples Hospital in Henan from Jan 1, 2003 to Dec 31, 2007. Results　During the five-year span, Escherichia coli, responsible for 44.8% of all UTIs, was the most predominant pathogen, followed by Enterococcus (17.7%), and then Candida albicans (11.5%). The Enterobacteriaceae was found 100% sensitive to imipenem, while Gram-positive Cocci were relatively sensitive to nitrofurantoin and vancomycin. Conclusion　Since the 1959 isolates from UTI patients were relatively highly resistant to ampicillin, piperacillin, ciprofloxacin, Levofloxacin and co-trimoxazoleto, these antibiotics should be used with caution. On the other hand, amikacin and nitrofurantoin are preferable. In brief, antibiotic therapy should rely rigorously on drug resistance surveillance due to high levels of drug resistance, even companied with multiple-drug resistance, of the pathogens.