浙江省舟山市海产品中副溶血性弧菌的定量检测

Quantitative measurement of seafood <I>Vibrio parahaemolyticus</I> in Zhoushan city, Zhejiang province

摘要: 目的　调查舟山市海产品中副溶血性弧菌（VP）的污染情况。方法　分3个季度，从舟山市不同的农贸市场采购鱼类、虾类等海产品作为检测对象，采用稀释培养计数（MPN）法对其中所含的VP进行定量检测，同时对分离到的VP进行毒力基因检测。结果　不同月份VP在鱼、虾等海产品中的含量呈明显的波动，分离到的33株VP中有2株副溶血性弧菌耐热溶血素阳性。结论　舟山市海产品中VP污染严重，应引起足够的重视。

Abstract: Objective　The study was conducted to identify the contamination of IVibrio parahaemolyticus/I in sea foods. Methods Seafood such as fish and shrimps purchased from individual farm markets in Zhoushan city in three separated quarters aresampled and analyzed. IVibrio parahaemolyticus/I was quantitatively identified using most probable numbers(MPN), and isolateswere detected for toxin genes. Results　The density of IVibrio parahaemolyticus/I in seafood varied significantly on a monthlybasis. TDH genes were detected in 2 out of 33 isolates of IVibrio parahaemolyticus/I. Conclusion　Heavy IVibrio parahaemolyticus/Icontamination was found in sea products in Zhoushan, requiring urgent attention.