Abstract: Objective To analyze the infection rate, drug resistance of the Escherichia coli and the drug resistance of extended-spectrum β-lactamases (ESBLs)-producing strain in the blood of the hospitalized patients from 2005 to 2000, provide the reference for clinical antibiotics use. Methods Blood were cultured with BacT/ALERT 3D, antibiotics susceptibility and ESBLs-producing strain were detected with Vitek-60, GNI + and GNS-143. Results A total of 132 strains of Escherichia coli were detected from the 9513 samples with 67 ESBLs-producing strains, the detection rate of ESBLs-producing strain was 50.8%. The antibiotic resistance rate of ESBLs-producing strain were much higher than that of non-ESBLs-producing strain. The resistance rate of ESBLs-producing strain to β-lactam antibiotics was 100%, and the resistant rates to quinolones, aminoglycosides and sulfonamides were very high. The resistance rates to cefotetan, piperacillin/tazobactam were 6.0% and 9.0% respectively, all strains were sensitive to imipenem. Conclusion Drug resistance of Escherichia coli in blood was serious, and the detection rates of ESBLs-producing strain were high, the antibiotics used in clinical treatment should be chosen according to the results of drug susceptibility test and ESBLs detection.