Abstract: ObjectiveTo isolate and culture Vibrio cholerae strains from stool specimens and determine its virulence genes and drug sensitivity. MethodsAfter conventional isolation, the strains were identified according to the biochemical and serological results. Polymerase chain reaction (PCR) was performed to detect such virulence genes as IctxA, ace, tcpA, zot./I Drug sensitivity was identified using K-B method. ResultsOne strain of El Tor biotype 6f of Vibrio cholerae was isolated from the stools of patients, which contained four kinds of virulence gene and was multi-drug-sensitive and non-producing -lactamase (ESBL). ConclusionThe results suggest that the isolate of IVibrio cholerae/I was the cause of pandemic, which was sensitive to a variety of commonly used antibiotics. PCR is an effective and fast approach for detection of the virulence strength of Vibrio cholerae.