中国羊种布鲁氏菌&lt;I&gt;omp2&lt;/I&gt;基因PstⅠ酶切多态性特征分析

姜海; 崔步云; 赵鸿雁; 朴东日; 李兰玉

doi:10.3784/j.issn.1003-9961.2009.04.013

中国羊种布鲁氏菌omp2基因PstⅠ酶切多态性特征分析

Analysis on PstⅠ digestion polymorphism of omp2 gene of Brucella melitensis isolated in China

摘要

摘要: 目的拟发现中国羊种布鲁氏菌Iomp2/I基因PstⅠ酶切多态性特征。方法用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对羊种标准菌株、疫苗株M5、国内分离的羊种布鲁氏菌野毒株及非典型株共46株进行分析。结果Iomp2/I基因扩增产物经PstⅠ酶切后呈现两种酶切图谱，43/46有238 bp和44 bp 2个条带；仅羊1型标准株16M和两株青海非典型羊种1型菌有282 bp、238 bp和44 bp 3个条带。结论Iomp2/I基因PstⅠ酶切可为区分部分野生动物中的羊种菌和家畜羊种菌，为揭示布鲁氏菌的遗传衍化关系提供线索。

Abstract: ObjectiveTo explore PstⅠ digestion polymorphism of Iomp2/I gene of IBrucella melitensis/I isolated in China. MethodsStandard strain of IBrucella melitensis/I, vaccine strain (M5), wild strain of IBrucella melitensis/I isolated in China and atypical strain were analyzed by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). ResultsThe amplification product of Iomp2/I gene had two PstⅠ digestion pattern. Of the 46 strains analyzed, 43 had 2 straps of 238 bp and 44 bp, only 1 standard strain of IBrucella melitensis/I biotype 1 (16M) and 2 atypical strains of IBrucella melitensis/I biotype 1 isolated in Qinghai had 3 straps of 282 bp、238 bp and 44 bp. ConclusionPstⅠ digestion of Iomp2/I gene can distinguish some IBrucella melitensis/I in wild animal and IBrucella melitensis/I in cattle, which provide the evidence to reveal the geno-derivation of IBrucella./I

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中国羊种布鲁氏菌<I>omp2</I>基因PstⅠ酶切多态性特征分析

Analysis on PstⅠ digestion polymorphism of <I>omp2</I> gene of <I>Brucella melitensis</I> isolated in China