Abstract: ObjectiveTo investigate drug resistance of IClostridium difficile/I isolated from hospital by PCR ribotyping and drug resistance detection to 8 clinically isolated strains of IClostridium difficile/I which were toxin producing. MethodsEight strains of toxin producing IClostridium difficile/I were isolated from clinical stool samples by biochemical identification and toxin detection, then the PCR ribotyping and the detection of minimum inhibition concentration (MIC) of ampiciline, clindamycin, metronidazole, vancomycin by Etest to the strains were conducted respectively, and the drug resistance was analyzed. ResultsOf 8 strains toxin producing IClostridium difficile/I, 5 belonged to a ribotype and 7 showed drug resistance, I.e. 3 were resistant to ampiciline, 7 were resistant to clindamycin, 1 was resistant to metronidazole, no strain was detected to be resistant to vancomycin. ConclusionThe drug resistance rate of IClostridium difficile/I isolated in hospital was high, and multi-drug resistance was observed. The drug susceptibility test of IClostridium difficile/I and other anaerobic bacterium should be strengthened in hospital.