假结核耶尔森菌毒力相关基因的检测及序列分析

Virulence genes detection and sequence analysis for Yersinia pseudotuberculosis

摘要: 目的对来自不同血清型的29株假结核耶尔森菌的主要毒力相关基因进行检测及序列分析，初步了解假结核耶尔森菌主要毒力基因的分布与变异情况。方法应用的聚合酶链反应（PCR）技术进行血清学分型与毒力基因（inv、pYV、HPI毒力岛、ypm）检测，并对ypm基因扩增基因进行测序比较。结果29株实验菌株均携带编码侵袭素的inv基因，15株携带毒力质粒（pYV），13株携带全部或部分HPIs基因簇，19株携带ypmA基因，1株携带ypmB基因，1株携带ypmC基因。结论假结核耶尔森菌毒力基因的分布具有一定的多态性。

Abstract: ObjectiveTo conduct virulence genes detection and sequence analysis for 29 strains of Yersinia pseudotuberculosis from different areas. MethodsPCR was used to detect the virulence factors of inv, pYV, HPI and ypm, and the sequencing was conducted for ypm. ResultsIt was found that all the 29 Y. pseudotuberculosis strains carried inv gene, 15 strains carried pYV, 13 strains carried all or part of HPIs, 19 carried ypmA, but ypmB was found in 1 strain and ypmC in another 1 strain. ConclusionThe distribution of the virulence genes in Y. Pseudotuberculosis showed geographical heterogeneity.