Abstract: Brucellosis is an important infectious disease which threatens human health and the development of animal husbandry, as well as a kind of biological weapon, which should be regarded as a worldwide public health problem. With the development of the research techniques of proteomics, which is technically supported by two dimensional gel electrophoresis, biological mass spectrogram, and bioinformatics, the research of proteome will further develop into a higher level. Recently, the proteomics research technique has been applied in the field of brucellosis study. This technique will not only establish theoretical basis for the study of regular pattern of brucella activity, but also provide important practical directions for the study on brucellas pathogenesis, diagnosis, disease control and vaccine development, which greatly contribute to the research of brucellosis.