黄泓滟, 王晓萍, 靳玉惠, 张文艳, 柯文鸿, 王海. 流行性脑脊髓膜炎病例实验室不同方法监测结果分析[J]. 疾病监测, 2009, 24(7): 495-497. DOI: 10.3784/j.issn.1003-9961.2009.07.008
引用本文: 黄泓滟, 王晓萍, 靳玉惠, 张文艳, 柯文鸿, 王海. 流行性脑脊髓膜炎病例实验室不同方法监测结果分析[J]. 疾病监测, 2009, 24(7): 495-497. DOI: 10.3784/j.issn.1003-9961.2009.07.008
HUANG Hong-yan, WANG Xiao-ping, JIN Yu-hui, ZHANG Wen-yan, KE Wen-hong, WANG Hai. Surveillance of epidemic cerebrospinal meningitis cases by different laboratory test[J]. Disease Surveillance, 2009, 24(7): 495-497. DOI: 10.3784/j.issn.1003-9961.2009.07.008
Citation: HUANG Hong-yan, WANG Xiao-ping, JIN Yu-hui, ZHANG Wen-yan, KE Wen-hong, WANG Hai. Surveillance of epidemic cerebrospinal meningitis cases by different laboratory test[J]. Disease Surveillance, 2009, 24(7): 495-497. DOI: 10.3784/j.issn.1003-9961.2009.07.008

流行性脑脊髓膜炎病例实验室不同方法监测结果分析

Surveillance of epidemic cerebrospinal meningitis cases by different laboratory test

  • 摘要: 目的应用实验室免疫学、病原学方法监测合肥市流行性脑脊髓膜炎(流脑)病例感染状况和菌群分布,比较不同方法监测结果。方法采集流脑疑似病例急性期和恢复期血液、急性期脑脊液,实验室诊断方法包括免疫学血清抗体检测、脑脊液抗原检测,病原学血液脑膜炎奈瑟菌(INm/I)培养、脑脊液INm/I培养,病原学血清INm/I的DNA特异片段检测。结果2005-2008年流脑疑似病例421份,实验室监测病例311份,免疫学血清抗体检测方法病例阳性率最高72.4%,其中C群流脑病例阳性率70.9%;临床诊断病例391份,临床诊断病例率92.9%,实验室诊断病例181份,实验室诊断病例率43.0%。结论合肥市流脑监测病例中流脑感染以C群INm/I为主;流脑病例实验室诊断与临床诊断之间存在差距,实验室诊断需要结合多种方法,加强质量管理。

     

    Abstract: ObjectiveTo conduct laboratory surveillance of epidemic cerebrospinal meningitis (ECM) cases in Hefei by using immunology and etiology tests and compare the surveillance results of different methods. MethodsThe blood samples of suspected ECM cases in acute phase and convalescence phase and the cerebrospinal fluid (CSF) samples in acute phase were taken. Laboratory diagnosis included immunology tests of serum antibody detection and CSF antigen detection and etiology tests of INeisseria meningitidis/I (INm/I) culture of blood sample, INm/I culture of CSF and INm/I DNA specific fragment detection. ResultsDuring 2005-2008, there were 421 suspected ECM cases, 311 were under laboratory surveillance. The positive rate of serum antibody detection was highest (72.4%), and the case of serogroup C accounted for 70.9% of the total cases. 391 cases were diagnosed clinically (92.9%), 181 cases were diagnosed by laboratory test (43.0%). ConclusionECM cases of serogroup C accounted for the largest proportion of the cases under surveillance in Hefei; there was difference between laboratory diagnosis and clinical diagnosis. Laboratory diagnosis should include more than one assay, and the quality control of the test should be strengthened.

     

/

返回文章
返回