Abstract: ObjectiveTo evaluate the field application of gold immunchromatography assay (GICA) in brucellosis surveillance area. MethodsGICA and rose bengal precipitation test (RBPT) were used to conduct parallel detection of sera from the persons who were at risk of brucellosis and in surveillance area and the cows in dairy farm where brucellosis had occurred. ResultsThe positive rates of two methods for human were 15.3% and 11.4% respectively, the difference had no statistical significance (P＞0.05), and the positive rates of two methods for cow were 14.4% and 16.0% respectively, the difference had no statistical significance (P＞0.05). ConclusionBoth GICA and RBPT are sensitive, but GICA is simple, rapid and requires small amount of sample, which is suitable for the field application in brucellosis surveillance area.