Abstract: Objective To evaluate the quality of non-remunerated blood donation by people with regular work in Jiangshan, Zhejiang province and provide evidence for the selection of low risk blood donors. Methods A total of 7775 people who donated blood during 2010-2012 in Jiangshan were selected to detect their HBsAg, antibody to hepatitis C (anti HCV), antibody to Treponema (anti TP) and antibody to HIV (anti HIV) with enzyme immunoassay system and detect their alanine aminotransferase (ALT) with automatic biochemical analyzer Results In recent three years 6.41% of blood donors was unqualified in Jiangshan, but this proportion was significantly lower in 2011 and 2012 than in 2010. (2= 202.9, P0.01), but differences in the proportion of blood donors with abnormal ALT were statistical significant (2=36.5, P0.01). The difference in overall unqualified proportion between male blood donors and female blood donors had statistical significance (2 = 11.7, P0.01). The differences in proportion of blood donors who were anti-HCV positive or with abnormal ALT between males and females had statistical significance (2=40.4, 11.9, P0.01), but the differences in other proportions of blood donors with unqualified detection results between males and females had no statistical significance (P0.05); The blood donors with abnormal ALT accounted for 59.7% of the total blood donors with unqualified detection results (298/499). The age specific differences in blood donors who were anti-TP, HBsAg positive or with abnormal ALT had statistical significance (2=19.4, 233.3 and 38.9, P0.01), but the age differences in blood donors who were anti HCV and anti HIV positive had no statistical significance (P0.05). The occupation specific differences in blood donors who were anti TP and HBsAg positive or with abnormal ALT had statistical significance (2=17.1, 13.2 and 24.1, P0.01), but had no statistical significance in blood donors who were anti HCV positive (2=2.3, P0.05). Conclusion It is necessary to conduct targeted health education, strengthen the counsel before blood donation and blood screening to select low risk blood donors and insure blood transfusion safety.