林淑银, 郭宝羡, 洪照宽, 陈爱平, 吴琳璇. 福建省漳州市海、水产品霍乱弧菌污染状况调查分析[J]. 疾病监测, 2010, 25(6): 456-458. DOI: 10.3784/j.issn.1003-9961.2010.06.010
引用本文: 林淑银, 郭宝羡, 洪照宽, 陈爱平, 吴琳璇. 福建省漳州市海、水产品霍乱弧菌污染状况调查分析[J]. 疾病监测, 2010, 25(6): 456-458. DOI: 10.3784/j.issn.1003-9961.2010.06.010
LIN Shu-yin, GUO Bao-xian, HONG Zhao-kuan, CHEN Ai-ping, WU Lin-xuan. Vibrio cholera contamination of marine and aquatic products in Zhangzhou city[J]. Disease Surveillance, 2010, 25(6): 456-458. DOI: 10.3784/j.issn.1003-9961.2010.06.010
Citation: LIN Shu-yin, GUO Bao-xian, HONG Zhao-kuan, CHEN Ai-ping, WU Lin-xuan. Vibrio cholera contamination of marine and aquatic products in Zhangzhou city[J]. Disease Surveillance, 2010, 25(6): 456-458. DOI: 10.3784/j.issn.1003-9961.2010.06.010

福建省漳州市海、水产品霍乱弧菌污染状况调查分析

Vibrio cholera contamination of marine and aquatic products in Zhangzhou city

  • 摘要: 目的 查明福建省漳州市海、水产品霍乱弧菌污染状况及菌型特征。 方法 2008年5-10月采集养殖场及市场、餐厅销售的海、水产品,用涂抹法对其体表、腮部和泄殖腔取样后,直接接种于碱性蛋白胨水进行增菌,划线接种庆大琼脂平板分离培养。取纯培养物进行染色镜检,用霍乱弧菌混合多价、O139群及单价分型诊断血清做玻片凝集试验,阳性菌株进一步做试管凝集及系统生化鉴定;使用常规PCR检测霍乱弧菌肠毒素ctxA基因。霍乱弧菌检出率的比较用2检验。 结果 漳州市海、水产品霍乱弧菌带菌率为5.08%(26/512),其中小川型15株、稻叶型11株。蛙类带菌率最高为25.00%(13/52)。ctxA基因检测均为阴性。增菌6~8 h霍乱弧菌检出率为0.78%、增菌18~24 h为2.34%,两种增菌时间霍乱弧菌检出率的比较差异有统计学意义(P0.05);第一次增菌霍乱弧菌检出率为2.34%、第二次为5.08%,两次增菌霍乱弧菌检出率的比较差异有统计学意义(P0.05)。 结论 漳州市海、水产品存在霍乱弧菌污染,需采取相应的预防措施防止霍乱疫情的发生。本次通过对染菌样品培养条件进行对比实验证明,染菌样品最适增菌时间应为18~24 h并宜二次增菌。

     

    Abstract: Objective To identify Vibrio cholera contamination of marine and aquatic products in Zhangzhou city as well as the bacterial types. Methods Smears were taken from the surface, gill and cloaca of marine and aquatic products sold in fisheries, markets and restaurants, which were directly inoculated into alkaline peptone water to allow bacterial growth. The resulting samples were streaked on gentamicin agar plates for isolation. Pure culture was drawn for staining microscopy. Slide agglutination tests were carried out using a mixture of Vibrio cholera and type-specific polyvalent, group O139 and monovalent sera, and positive strains were subject to tube agglutination and systematic biochemical identification. Conventional PCR was performed to identify the Vibrio cholera enterotoxin gene ctxA. The detection rates of Vibrio cholera were compared using the chi-square test. Results The Vibrio cholera-carrying rate in marine and aquatic products in Zhangzhou was 5.08% (26/512). Fifteen strains of Ogawa serotype and 11 of Inaba serotypes were found. The highest bacteria-carrying rate was found in frogs(25.00%,13/52). No ctxA-bearing strains were detected. After 6 to 8 hours of bacterial growth, the detection rate of Vibrio cholera was 0.78%, which increased to 2.34% after 18 to 24 hours of growth. The difference between the two bacterial growth times was significant (P0.05). The detection rate increased to 5.08% after a second growth from 2.34% of the first, which was significantly different (P0.05). Conclusion Vibrio cholera contamination of marine and aquatic products was fououdn in Zhangzhou city, entailing appropriate control measures to prevent the occurrence of cholera epidemics. Comparative experiments on the culture conditions of contaminated samples revealed that the optimum time for bacterial growth should be 18-24 h with a preferably additional growth.

     

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