Abstract: Campylobacter jejuni is recognized as one of the major causes of human food-borne diseases. Developing a rapid and specific detection method is important for the infection diagnosis and pathogen surveillance.Real-time PCR, as one of the rapid and sensitive methods, has been widely used to detect and identify Campylobacter jejuni. The aim of this review is to introduce and summarize the establishment, development and application of this method in Campylobacter jejuni.