25株副溶血性弧菌分子流行病学特征分析

Molecular epidemiological characteristics of 25 strains of Vibrio parahemolyticus

摘要: 目的运用荧光PCR技术和脉冲场凝胶电泳(PFGE)技术对成都市2009年分离的25株副溶血性弧菌进行毒素基因检测和分子分型,分析感染来源。方法利用PCR检测副溶血性弧菌分离株耐热直接溶血素(tdh)基因和耐热相关溶血素(trh)基因。用PFGE对菌株进行分型,所得结果用BioNumerics V 4.0软件UPGMA方法进行聚类分析。结果所试25株分离菌tdh基因阳性有20株,1株为trh阳性。以SfiⅠ酶切后PFGE分型,25株菌被分成了16个带型,环境分离株菌型分散。结论成都市副溶血性弧菌暴发的感染来源复杂,PFGE技术可以对暴发流行中的细菌进行分析鉴定。

Abstract: Objective To analyze Vibrio parahemolyticus isolated from the outbreaks in Chengdu during 2008 - 2009 by using pulsed field gel electrophoresis (PFGE) and PCR typing, and trace the infection sources. Methods Virulence genes tdh and trh were detected by PCR, PFGE was used for subtyping of isolates. Patterns were compared by using the software BioNumerics. Results Of the 25 isolates,20 were tdh positive and 1 were trh positive. The 25 strains were clustered into 16 PFGE patterns when digested with SfiⅠ. The PFGE patterns of isolates from the environment varied. Conclusion The infection sources of the outbreaks in Chengdu were complex. PFGE may be used to analyze and identify isolates in outbreak.