绵羊附睾种布鲁氏菌019株外膜蛋白基因序列比较研究

Comparative study on omp gene sequences of Brucella ovis 019 strain

摘要: 目的证实新疆分离绵羊附睾种布鲁氏菌019株与参考菌株的差异性。方法采用PCR扩增绵羊附睾种布鲁氏菌019株外膜蛋白的Omp31、Omp25、Omp22、Omp2a、Omp2b基因,并进行序列比较分析。结果绵羊附睾种布鲁氏菌019株的5种外膜蛋白基因序列与绵羊种布鲁氏菌参考株在核苷酸水平和氨基酸水平上均存在差异。结论绵羊附睾种布鲁氏菌019株是独特的新疆地方株。

Abstract: Objective To prove the difference between Brucella ovis 019 strain isolated in Xinjiang and reference strain. Methods The Omp31、Omp25、Omp22、Omp2a、Omp2b gene sequences of Brucella ovis 019 strain were analyzed by polymerase chain reaction and DNA sequencing. Results There was significant difference in either DNA or amino level between Brucella ovis 019 strain and 63/290 reference strain; Conclusion Brucella ovis 019 strain is an unique local strain in Xinjiang.