Abstract: Objective To analyze the detection of infectious pathogens and their drug resistance in intensive care unit (ICU) in Wenzhou Integrated Chinese and Western Medicine Hospital, and provide evidence for the rational antibiotic use. Methods The statistical analysis was conducted on the clinical data of infection cases in the ICU from January 2007 to December 2009, including 2019 cases in 2007,2801 cases in 2008 and 2300 cases in 2009. Results From 2007 to 2009, the detection rate of Pseudomonas aeruginosa was highest. Fungal infection accounted for about 20% of total infection cases. The detection rate of Serratia marcescens was 4.8% in 2007,4.9% in 2008 and 5.7% in 2009. The detection rate of Acinetobacter baumannii was 6.8% in 2007,10.6% in 2008 and 5.7% in 2009. G-bacilli were highly sensitive to cefoperazone/sulbactam. The resistance to 3rd and 4th generation cephalosporins increased greatly by years. The resistant rate of Pseudomonas aeruginosa to carbapenem antibiotics was about 60%. The resistant rate of Serratia marcescens to meraopenem was 2.0% in 2007,64.2% in 2008 and 43.2% in 2009. Similar resistance was observed for imipenem. The resistant rate to piperacillin/tazobactam was as high as 80% during 2008 - 2009. The detection rate of MRSA was 1.3% in 2007,2.2% in 2008 and 5.0% in 2009, but no strains being resistant to vancomycin, teicoplanin and linezolid had been detected. Conclusion In the ICU, Pseudomonas aeruginosa infection was most serious, the detection and drug resistant rates of Serratia marcescens increased by years, and the resistant rates of Pseudomonas aeruginosa and Serratia marcescens to carbapenem antibiotics were as high as 50%. It is necessary to strengthen the surveillance of drug resistant strains and use antibiotics rationally.