屠丽红, 陈洪友, 陈敏. 利用脉冲场凝胶电泳及核糖体分型技术对14株上海崇明地区O139群霍乱弧菌分型分析[J]. 疾病监测, 2011, 26(2): 159-160,168. DOI: 10.3784/j.issn.1003-9961.2011.02.025
引用本文: 屠丽红, 陈洪友, 陈敏. 利用脉冲场凝胶电泳及核糖体分型技术对14株上海崇明地区O139群霍乱弧菌分型分析[J]. 疾病监测, 2011, 26(2): 159-160,168. DOI: 10.3784/j.issn.1003-9961.2011.02.025
TU Li-hong, CHEN Hong-you, CHEN Min. Study on 14 Vibrio cholerae O139 strains by PFGE and ribotyping in Chongming, Shanghai[J]. Disease Surveillance, 2011, 26(2): 159-160,168. DOI: 10.3784/j.issn.1003-9961.2011.02.025
Citation: TU Li-hong, CHEN Hong-you, CHEN Min. Study on 14 Vibrio cholerae O139 strains by PFGE and ribotyping in Chongming, Shanghai[J]. Disease Surveillance, 2011, 26(2): 159-160,168. DOI: 10.3784/j.issn.1003-9961.2011.02.025

利用脉冲场凝胶电泳及核糖体分型技术对14株上海崇明地区O139群霍乱弧菌分型分析

Study on 14 Vibrio cholerae O139 strains by PFGE and ribotyping in Chongming, Shanghai

  • 摘要: 目的 对14株上海市崇明地区2005年O139群霍乱弧菌菌株进行脉冲场凝胶电泳(PFGE)及核糖体分型分析。 方法 用PFGE、核糖体分型对霍乱弧菌进行分析。 结果 14株O139群霍乱弧菌产毒株经核糖体分型分析为同一型;而PFGE双酶切分型结果为其中10株为同一型,其余4株为高度同源性。 结论 上海市崇明地区2005年流行的O139群霍乱弧菌核酸指纹分型基本一致。

     

    Abstract: Objective To understand the genotypes of 14 strains of Vibrio cholerae O139 isolated in Chongming in 2005. Methods Pulsed-field gel electrophoresis (PFGE) and ribotyping were conducted to analyze the Vibrio cholerae strains. Results Ribotyping indicated that the genotypes of all the O139 strains were same; but PFGE indicated that 10 of 14 strains of Vibrio cholerae O139 belonged to same type, the other 4 strains were highly homologous. Conclusion The genotypes of Vibrio cholerae O139 circulating in Chongming in Shanghai in 2005 were similarly.

     

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