Abstract: Objective To establish a rapid detection assay of Streptococcus suis serotype 2 with Taqman-MGB probe based on real-time PCR technology. Methods By using Beacon Designer 7.0, primers and Taqman-MGB probe were designed and the real-time PCR detection assay was established to detect the csp2J gene sequence of Streptococcus suis serotype 2. The target fragment was cloned into the pMD18-T vector to make standard curve. The specificity of the probe was verified with common and conditional pathogenic bacteria, blood simulating samples were prepared to evaluate the clinical application of this assay. Results The standard curve showed that the 100 copies csp2J gene per reaction could be detected by this assay, and specificity detection revealed that only the reaction of Streptococcus suis type 2 showed the fluorescence signal, while that of other common and conditional pathogenic bacteria showed no fluorescence signal, and the lowest testing limitation of blood simulating samples was 3.9102CFU per ml. Conclusion The real-time PCR detection assay of Streptococcus suis serotype 2 with csp2J as target gene is sensitive, specific and rapid, which can be used in preliminary screening of samples of patients infected with Streptococcus suis type 2.