Abstract: Objective To understand the incidence and drug resistance of pulmonary tuberculosis (TB) in floating population in Jiangbei district and provide scientific evidence for the prevention and control of TB. Methods The analysis was conducted on the incidence data of pulmonary TB reported by Jiangbei CDC from 2008 to 2010, and the drug susceptibility tests of smear positive strains to isoniazide, rifampin, streptomycin and ethambutol were conducted. Results The pulmonary TB cases in floating population accounted for 50.6% of the total in Jiangbei, and most cases were young workers and migrant workers (68.8%). The cases were mainly found through medical care seeking, accounting for 65.2%. In the TB cases in floating population, the overall drug resistant rate was 37.1%, the multi-drug resistant rate was 12.9%, the drug resistant rate in initial treatment was 32.1% and acquired drug resistant rate was 66.7%. The resistant rates to rifampin, isoniazid, streptomycin and ethambutol were 12.9%, 19.4%, 21.1%, and 6.5% respectively. Conclusion Pulmonary TB cases mainly occurred in floating population in Jiangbei, and the drug resistance was serious. It is necessary to strengthen the prevention and control of TB in floating population.