戴广明, 黄海荣, 梁倩, 姜广路, 逄宇, 周杨, 王胜芬, 赵雁林, 李卫民. 新疆维吾尔自治区与甘肃省部分地区结核分枝杆菌分子流行病学特征比较研究[J]. 疾病监测, 2011, 26(8): 592-597,613. DOI: 10.3784/j.issn.1003-9961.2011.08.003
引用本文: 戴广明, 黄海荣, 梁倩, 姜广路, 逄宇, 周杨, 王胜芬, 赵雁林, 李卫民. 新疆维吾尔自治区与甘肃省部分地区结核分枝杆菌分子流行病学特征比较研究[J]. 疾病监测, 2011, 26(8): 592-597,613. DOI: 10.3784/j.issn.1003-9961.2011.08.003
DAI Guang-ming, HUANG Hai-rong, LIANG Qian, JIANG Guang-lu, PANG Yu, ZHOU Yang, WANG Sheng-fen, ZHAO Yan-lin, LI Wei-min. Comparison of molecular epidemiology of Mycobacterium tuberculosis in parts of Xinjiang and Ganshu provinces[J]. Disease Surveillance, 2011, 26(8): 592-597,613. DOI: 10.3784/j.issn.1003-9961.2011.08.003
Citation: DAI Guang-ming, HUANG Hai-rong, LIANG Qian, JIANG Guang-lu, PANG Yu, ZHOU Yang, WANG Sheng-fen, ZHAO Yan-lin, LI Wei-min. Comparison of molecular epidemiology of Mycobacterium tuberculosis in parts of Xinjiang and Ganshu provinces[J]. Disease Surveillance, 2011, 26(8): 592-597,613. DOI: 10.3784/j.issn.1003-9961.2011.08.003

新疆维吾尔自治区与甘肃省部分地区结核分枝杆菌分子流行病学特征比较研究

Comparison of molecular epidemiology of Mycobacterium tuberculosis in parts of Xinjiang and Ganshu provinces

  • 摘要: 目的 比较研究新疆维吾尔自治区与甘肃省部分地区结核分枝杆菌分子流行病学特征。 方法 2008年3-12月从新疆维吾尔自治区与甘肃省的3个县(区)收集痰涂片阳性的结核病患者的临床分离株。收集患者的性别、民族、病史、菌株耐药谱和菌株来源资料。药物敏感性实验采用比例法。运用实时荧光定量PCR(Realtime PCR)和结核分枝杆菌散在分布重复单位(MIRU-VNTR)基因分型方法鉴定北京基因型及亚型菌株并绘制系统发育树。 结果 在146份结核分枝杆菌分离株中,北京/W 系结核分枝杆菌84株,占57.5%。其中较现代的W菌/典型北京家族菌株66株,占78.6%。MIRU-VNTR基因分型方法将146株菌株分成140个基因型,其中包括134个独特型,6个成簇的基因型。 结论 在甘肃采样地区,北京/W 系的流行呈现明显优势,其中较为现代的W 菌/典型北京家族菌株的流行又呈较为明显的优势;而在新疆采样地区,以非北京/W 系菌株流行为主,两地结核分枝杆菌分子流行病学特征有明显区别。

     

    Abstract: Objective To understand the difference on molecular epidemiology of Mycobacterium tuberculosis in parts of Xinjiang and Ganshu provinces. Methods Clinical isolates were collected from smear positive TB patients in 3 counties (district) in Xinjiang and Gansu from March to December in 2008. The patients' gender, ethnic group, disease history, drug resistant spectrum and sample source information were collected too. Drug susceptibility test was performed by using proportion method. Beijing genotype and sub-genotypes of M. tuberculosis were identified by using real time PCR. The phylogenetic tree of M. tuberculosis was constructed by using MIRU-VNTR method. Results Among 146 M. tuberculosis clinical isolates, eighty-four were Beijing/W lineage strains (57.5%), of which 66 were modern W strains/typical Beijing strains. The MIRU-VNTR typing results showed that there were 140 genotypes in 146 strains, including 134 unique genotypes and 6 cluster genotypes. Conclusion Beijing/W lineage strains were predominant in the strains sampled in Gansu, and most of them were modern W strains/typical Beijing strains, but non Beijing/W lineage strains were predominant in the strains sampled in Xinjiang. The difference on molecular epidemiology of M. tuberculosis between Xinjiang and Gansu was obvious.

     

/

返回文章
返回