Abstract: Objective To understand the epidemiological characteristics of hemorrhagic fever with renal syndrome (HFRS) in Longquan from 2005 to 2010 and provide scientific evidence for the prevention and control of the disease. Methods Descriptive epidemiological analysis was conducted on the incidence data of HFRS in Longquan during this period. The HV-positive lung sample of mouse was inoculated to Vero-E6 cells to isolate virus. HV was identified by DFA using Anti-HV McAb. Results A total of 278 HFRS cases, including 2 deaths, were reported from 2005 to 2010, the annual average incidence was 16.38/lakh and the case fatality was 0.72%. The incidence peaks occurred during May-July and November-January, the cases during these two periods accounted for 31.65% and 37.05% of the total respectively. Three strains isolated were identified as Hantaan virus (serotype I). Conclusion It is evident that natural foci area of HFRS exists in Longquan in Zhejiang province, HTNV strain of Hantaviruses might be predominant. The prevention and control of the disease should be strengthened.