孙晓芳, 杨郝亮, 周海健, 朱兵清, 徐丽, 高源, 邵祝军. 中国脑膜炎奈瑟菌nadA基因多态性分析[J]. 疾病监测, 2012, 27(11): 844-848. DOI: 10.3784/j.issn.1003-9961.2012.11.003
引用本文: 孙晓芳, 杨郝亮, 周海健, 朱兵清, 徐丽, 高源, 邵祝军. 中国脑膜炎奈瑟菌nadA基因多态性分析[J]. 疾病监测, 2012, 27(11): 844-848. DOI: 10.3784/j.issn.1003-9961.2012.11.003
SUN Xiao-fang, YANG Hao-liang, ZHOU Hai-jian, ZHU Bing-qing, XU Li, GAO Yuan, SHAO Zhu-jun. Polymorphism of nadA gene of Neisseria meningitidis in China[J]. Disease Surveillance, 2012, 27(11): 844-848. DOI: 10.3784/j.issn.1003-9961.2012.11.003
Citation: SUN Xiao-fang, YANG Hao-liang, ZHOU Hai-jian, ZHU Bing-qing, XU Li, GAO Yuan, SHAO Zhu-jun. Polymorphism of nadA gene of Neisseria meningitidis in China[J]. Disease Surveillance, 2012, 27(11): 844-848. DOI: 10.3784/j.issn.1003-9961.2012.11.003

中国脑膜炎奈瑟菌nadA基因多态性分析

Polymorphism of nadA gene of Neisseria meningitidis in China

  • 摘要: 目的 了解我国脑膜炎奈瑟菌(Neisseria meningitidis,Nm)主要黏附蛋白(Neisseria adhesion A,NadA)编码基因nadA的多态性特征,以期研究我国主要流行Nm菌株NadA蛋白的流行病学意义及NadA蛋白作为脑膜炎球菌蛋白疫苗候选蛋白研发的可行性。 方法 选取我国26个省(市、自治区)1963-2012年间不同血清群代表性Nm菌株230株,采用多位点序列分型(muliti-locus sequence typing,MLST)、PCR、斑点杂交、基因测序等方法,检测Nm菌株基因型特征、nadA基因分布的多态性及其基因序列特征,利用BioEdit、Mega 4.0等软件对nadA基因序列进行比对分析。 结果 我国230株不同血清群的Nm菌株中,nadA+菌株55株,仅存在于ST-5 complex(序列群)的A群和ST-11 complex 的W135群Nm菌株中,B、C及其他血清群Nm菌株nadA-。nadA+菌株基因型以variant 3(V3)型为主(54/55),包括5种亚型(NadA V3.1~3.5),同源性高达99%以上。 结论 我国Nm菌株中,仅ST-5 complex的A群和ST-11 complex的W135群菌株存在NadA黏附蛋白,型别以variant 3型为主。B群菌株不含有NadA蛋白,variant 3型NadA蛋白不适合作为我国B群Nm(B meningococci,MenB)蛋白疫苗的候选蛋白。

     

    Abstract: Objective To understand the polymorphism of nadA gene of Neisseria meningitidis isolates in China, investigate the epidemiological significance of NadA protein of major N. meningitidis strains circulated in China and explore the feasibility of developing NadA protein based N. meningitidis vaccine. Methods Totally 230 N. meningitidis strains isolated in 26 provinces from 1963 to 2012 were analyzed by using muliti locus sequence typing(MLST), PCR, dot blotting assay and gene sequencing. The sequences of nadA genes were aligned by using BioEdit and Mega 4.0 software. Results Of the 230 N. meningitidis strains, only 55 were nadA positive. All the nadA positive N.meningitidis strains belonged to serogroup A(ST-5 complex) and serogroup W135(ST-11 complex). No nadA positive strains were found in serogroup B, C and others serogroups. NadA variant 3 included five different subvariants(NadA V3.1-3.5) with high homology up to 99%. Of 55 nadA positive N. meningitidis strains,54 were variant 3. Conclusion In China, NadA protein was only present in serogroup A(ST-5 complex) and serogroup W135(ST-11 complex) and absent in serogroup B of N. meningitidis isolates. Variants 3 NadA protein is not suitable for using as candidate protein of N. meningitidis B vaccine in China.

     

/

返回文章
返回