Abstract: Objective To understand the types of spa gene of methicillin resistant Staphylococcus aureus(MRSA) in Ruian, Zhejiang province. Methods MRSA strains were selected by K-B test from 100 Staphylococcus aureus strains isolated in our hospital from March to November 2011. The X region of the strains spa gene was amplified with PCR and genotyping was conducted after sequencing. Results Totally 48 MRSA strains were identified, which belonged to 16 genotypes, type t030 was detected in 15 strains, t437 in 12 strains, t6944 in 5 strains, t172 in 3 strains, t9538 in 2 strains and t5699, t148, t179, t1751, t015, t5554, t127, t159, t062 and t163 in 1 stain respectively. Meanwhile a new spa type(t10149) was detected. Conclusion The major spa types of MRSA are t030 and t437 in Ruian and t10149 is a new type.