Abstract: Objective To understand the infection status of HIV, TP, HCV and HBV and related risk factors among men who have sex with men (MSM) in Lucheng district, with men Wenzhou municipality, Zhejiang province, and provide scientific evidence for the prevention and control of the diseases. Methods With informed consent obtained, a cross sectional survey was conducted among MSM using structured anonymous questionnaire by well trained investigators and volunteers. The MSMs blood samples were collected to detect HBsAg and the antibodies to HIV, TP and HCV. Results A total of 401 MSM were investigated and their awareness rate of AIDS related knowledge was 72.32%. The condom use rates of MSM at each sex with males and females were 48.66% and 33.96% respectively during the past 6 months. Totally 32.55% of MSM had commercial anal sex with other males. The positive rates of HIV, TP, HCV and HBsAg were 9.23%, 10.47%, 0.25% and 3.24% respectively. Conclusion The infection rates of HIV and TP among MSM were relatively high and comprehensive behavior intervention on risk behaviors should be initialed among them as soon as possible.