可变数目串联重复序列在宁波地区结核分枝杆菌基因分型研究中的应用
Application of variable number tandem repeats in genotyping of Mycobacterium tuberculosis strains in Ningbo
-
摘要: 目的 应用可变数目串联重复序列(VNTR)分子分型技术,对宁波地区138株肺结核临床分离株进行分型研究,探讨宁波地区结核菌株DNA多态性和基因型特征。 方法 根据文献选取7个分型效果较好的VNTR基因位点,应用聚合酶链反应(PCR)和琼脂糖凝胶电泳,分析结核分枝杆菌DNA多态性。 结果 共对138株结核分枝杆菌的7个VNTR位点进行了检测,共产生7个基因簇和123个独立基因型,成簇率为5.8%,VNTR-7位点基因分型技术分辨率指数(HGI)为0.9991,VNTR-3820位点的多态性最高。 结论 宁波地区的结核分枝杆菌存在较高的基因多态性,且VNTR-7位点基因分型技术有较高的分辨力,适用于宁波地区结核病分子流行病学研究。Abstract: Objective To conduct genotyping of Mycobacterium tuberculosis strains isolated from the patients in Ningbo with a new method based on the variable number tandem repeats (VNTR). Methods Seven VNTR loci were selected and the polymorphism of DNA fingerprinting of M. tuberculosis was analyzed with polymerase chain reaction (PCR) and agarose gel electrophoresis. Results The 7 VNTR loci of 138 M. tuberculosis strains were detected, and 7 clusters and 123 distinct patterns were found. The HGI value of VNTR-7 was 0.9991, and the percentage of VNTR-7 was 5.8%. The VNTR-3820 loci had the polymorphism. Conclusion The polymorphism of the M. tuberculosis isolates was obvious in Ningbo. The method of VNTR-7 showed high discrimination power in the detection of M. tuberculosis, which is easy to use and suitable for the TB control program.
下载: