夏文, 薛琳, 周显凤, 樊国印, 贺凤兰, 甘启芦. 一起由肠侵袭性大肠埃希菌O136:K78引起的聚集性腹泻暴发[J]. 疾病监测, 2012, 27(9): 726-728. DOI: 10.3784/j.issn.1003-9961.2012.9.018
引用本文: 夏文, 薛琳, 周显凤, 樊国印, 贺凤兰, 甘启芦. 一起由肠侵袭性大肠埃希菌O136:K78引起的聚集性腹泻暴发[J]. 疾病监测, 2012, 27(9): 726-728. DOI: 10.3784/j.issn.1003-9961.2012.9.018
XIA Wen, XUE Lin, ZHOU Xian-feng, FAN Guo-yin, HE Feng-lan, GAN Qi-lu. An outbreak of diarrhea caused by enteroinvasive Escherichia coli O136:K78[J]. Disease Surveillance, 2012, 27(9): 726-728. DOI: 10.3784/j.issn.1003-9961.2012.9.018
Citation: XIA Wen, XUE Lin, ZHOU Xian-feng, FAN Guo-yin, HE Feng-lan, GAN Qi-lu. An outbreak of diarrhea caused by enteroinvasive Escherichia coli O136:K78[J]. Disease Surveillance, 2012, 27(9): 726-728. DOI: 10.3784/j.issn.1003-9961.2012.9.018

一起由肠侵袭性大肠埃希菌O136:K78引起的聚集性腹泻暴发

An outbreak of diarrhea caused by enteroinvasive Escherichia coli O136:K78

  • 摘要: 目的 明确聚集性食源性腹泻暴发的病原。 方法 根据现场流行病学调查,对患者肛拭子标本18份,粪便标本2份,厨师粪便标本1份,进行分离培养,对分离到的可疑病原菌进行生化鉴定,采用Real-time PCR技术检测可疑病原菌的ipaH毒力基因,同时进行脉冲场凝胶电泳(PFGE)分型分析。 结果 分离到11株血清型为O136:K78的肠侵袭性大肠埃希菌,除1份肛拭子外,其余7份患者肛拭子、3份粪便标本Real-time PCR均阳性;除1株菌外,其余10株菌株的PFGE带型一致。 结论 具有侵袭性相关基因ipaH的O136:K78肠侵袭大肠埃希菌是本次聚集性腹泻暴发的主要原因。

     

    Abstract: Objective determine the pathogen of a foodborne outbreak of diarrhea. Methods On the basis of epidemiological investigation, eighteen rectal swabs and 2 stool samples from the patients and 1 stool sample from a cook were collected. After pathogen isolation and culture, the biochemical identification of the isolated strains was performed,the invasive plasmid antigen H gene (ipaH) of the positive strains were detected by real-time PCR and the pulsed-field gel electrophoresis homology analysis was conducted. Results Eleven strains of enteroinvasive Escherichia coli O136:K78 were isolated. All the samples, except 1 rectal swab, were positive in real time PCR, and the PFGE patterns of all the strains, except one, were consistent. Conclusion The enteroinvasive Escherichia coli O136:K78 with ipaH gene was the pathogen to cause the outbreak of diarrhea.

     

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