李婵媛. 2012年辽宁省铁岭市食源性致病菌监测中检测出1例诺如病毒[J]. 疾病监测, 2012, 27(9): 729-731. DOI: 10.3784/j.issn.1003-9961.2012.9.019
引用本文: 李婵媛. 2012年辽宁省铁岭市食源性致病菌监测中检测出1例诺如病毒[J]. 疾病监测, 2012, 27(9): 729-731. DOI: 10.3784/j.issn.1003-9961.2012.9.019
LI Chan-yuan. Detection of norovirus in foodborne pathogen surveillance in Tieling, Liaoning, 2012[J]. Disease Surveillance, 2012, 27(9): 729-731. DOI: 10.3784/j.issn.1003-9961.2012.9.019
Citation: LI Chan-yuan. Detection of norovirus in foodborne pathogen surveillance in Tieling, Liaoning, 2012[J]. Disease Surveillance, 2012, 27(9): 729-731. DOI: 10.3784/j.issn.1003-9961.2012.9.019

2012年辽宁省铁岭市食源性致病菌监测中检测出1例诺如病毒

Detection of norovirus in foodborne pathogen surveillance in Tieling, Liaoning, 2012

  • 摘要: 目的 了解辽宁省铁岭市水产品中食源性致病菌的污染状况,有效预防控制食源性疾病的发生。 方法 应用反转录-聚合酶链反应(RT-PCR)对2012年第一季度食源性致病菌常规监测采集到的6份水产品进行检测,对阳性PCR产物进行基因测序。 结果 6份水产品中,有1份检测出诺如病毒RNA阳性。测序结果分析显示,该阳性标本的诺如病毒VP1全基因序列测定和分析显示为GⅡ-4型诺如病毒。 结论 铁岭市首次在食源性致病菌日常监测中检测出诺如病毒GⅡ-4型。因此应加大对生食水产品的监督检查力度,避免因诺如病毒引起的病毒性胃肠炎暴发。

     

    Abstract: Objective understand the contamination status of foodborne pathogens in aquatic products in Tieling for the effective prevention and control of foodborne diseases. Methods Reverse transcription-polymerase chain reaction (RT-PCR).was conducted to detect 6 aquatic product samples collected during the routine surveillance in Tieling in the first quarter, 2012,and gene sequencing was done for the positive PCR products. Results Norovirus was detected in 1 of the 6 aquatic product samples and the sequence analysis indicated that it belonged to GⅡ-4 genotype. Conclusion It was the first time to detect norovirus GⅡ-4 genotype in the routine surveillance of foodborne pathogens in Tieling. It is necessary to strengthen the inspection and detection of aquatic products to prevent the outbreak of norovirus related viral gastroenteritis.

     

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