朱俊洁, 王鹏, 张蓉, 海荣, 梁莹, 宋志忠. 云南省鼠疫耶尔森菌多位点可变数目串联重复序列分析[J]. 疾病监测, 2013, 28(10): 848-852. DOI: 10.3784/j.issn.1003-9961.2013.10.016
引用本文: 朱俊洁, 王鹏, 张蓉, 海荣, 梁莹, 宋志忠. 云南省鼠疫耶尔森菌多位点可变数目串联重复序列分析[J]. 疾病监测, 2013, 28(10): 848-852. DOI: 10.3784/j.issn.1003-9961.2013.10.016
ZHU Jun-jie, WANG Peng, ZHANG Rong, HAI Rong, LIANG Ying, SONG Zhi-zhong. Multiple-locus variable-number tandem-repeat analysis on Yersinia pestis in Yunnan[J]. Disease Surveillance, 2013, 28(10): 848-852. DOI: 10.3784/j.issn.1003-9961.2013.10.016
Citation: ZHU Jun-jie, WANG Peng, ZHANG Rong, HAI Rong, LIANG Ying, SONG Zhi-zhong. Multiple-locus variable-number tandem-repeat analysis on Yersinia pestis in Yunnan[J]. Disease Surveillance, 2013, 28(10): 848-852. DOI: 10.3784/j.issn.1003-9961.2013.10.016

云南省鼠疫耶尔森菌多位点可变数目串联重复序列分析

Multiple-locus variable-number tandem-repeat analysis on Yersinia pestis in Yunnan

  • 摘要: 目的 通过多位点可变数目串联重复序列分析(multiple-locus variable-number tandem-repeat analysis,MLVA)方法,对云南省鼠疫菌株进行基因分型。 方法 采用聚合酶链反应(PCR)和毛细管电泳,通过BioNumerics软件进行处理,分析云南省158株鼠疫耶尔森菌基因型。 结果 选取鼠疫菌的14个VNTR位点,5个VNTR位点对云南省鼠疫菌具有多态性分析意义,利用这5个位点对云南菌株进行分析,云南158株鼠疫菌可分为2个群,3个簇,5个基因型,野鼠鼠疫菌属于A簇,丽江玉龙鼠疫菌属于B簇,家鼠鼠疫菌属于C簇,与传统的生态分型结果吻合。 结论 本次试验筛选的5个位点可以用于云南省鼠疫菌的分型,云南家鼠鼠疫菌、野鼠鼠疫菌及玉龙鼠疫菌分属不同的基因簇,玉龙鼠疫菌与野鼠鼠疫菌同属一个群,聚类结果与实际的地理相关性很好。

     

    Abstract: Objective To identify genotypes of Yersinia pestis in Yunnan by using Multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA). Methods By using polymerase chain reaction (PCR) and capillary electrophoresis, the genotypes of 158 Yersinia pestis strains isolated in Yunnan province were analyzed, and the data were processed with BioNumerics software. Results Five VNTR sites can be used to identify the genotypes of Yersinia pestis in Yunnan. The clustering of genotype showed that these strains could be classified into 2 groups, 3 clusters and 5 genotypes. The wild rodent strains belonged to cluster A, the stains isolated in Yulong belonged to cluster B and commensal rodent strains belonged to cluster C. The Results were consistent with traditional ecological classification. Conclusion The wild rodent strains, commensal rodent strains and Yulong strains isolated in Yunnan belonged to different gene clusters, but Yulong strains and wild rodent strains were in one group. The clustering analysis showed good relationship between the genotypes of Yersinia pestis and geographic location.

     

/

返回文章
返回