Abstract: Objective To investigate the distribution and drug resistance of Enterococci in patients with urinary tract infection and provide evidence for the rational use of antibiotics. Methods The urine specimen was cultured in Uricult. The identification of clinical isolates of Enterococci and their drug susceptibility test were conducted with ATB-Expression system, and the data were analyzed with Whonet 5.6 software. Results A total of 238 Enterococcus strains were detected, including 122 strains of E. faecalis (51.3%) and 95 strains of E. faecium (39.9%). The strains were mainly detected in department of internal medicine (38.2%) and department of urology (21.0%). The resistance rate of the Enterococcus strains was 86.5% to erythromycin, 75.7% to rifampin, 66.4% to tetracycline, 19.7% to nitrofurantoin, 9.6% to vancomycin and 0.0% to linezolid. The resistant rates of E. faecalis strains were 76.5% to rifampicin, erythromycin, quinupristin/dalfopristin and tetracycline, 0.9% to vancomycin. The resistant rates of E. faecium strains were 75.8% to ampicillin and other antibiotics and 4.3% to vancomycin. The resistant rates of E. faecalis strains to most antibiotics were lower than E. faecium strains, except tetracycline, quinupristin/dalfopristin. The differences in resistant rates to rifampicin, vancomycin and linezolid between E. faecium and E. faecalis had no statistical significance; Compared with 2007, the resistant rates of Enterococci to penicillin, ampicillin and other antibiotics increased in 2012, but the resistant rates to ciprofloxacin and tetracycline declined. Conclusion Enterococci are the major pathogens causing urinary tract infection, mainly including E. faecalis and E. faecium. The resistant rates vary greatly with different Enterococci. It is important to enhance bacterial culture and regular analysis of drug resistance to improve clinical cure rate and rational use of antibiotics.