Abstract: Objective To understand the distribution and drug resistance of Staphylococcus aureus strains isolated in Fuyang People's Hospital, Zhejiang province, from 2009 to 2011,and provide evidence for the rational use of antibiotics. Methods S. aureus strains were isolated from the clinical samples collected from the patients in our hospital and identified with ATB.The drug susceptibility test of the strains was conducted by using K-B method. D-test recommended by CLSI was conducted to detect erythromycin induced clindamycin resistance. Results A total of 225 S. aureus strains were isolated during this period. The isolation rate in sputum or bronchial aspirate was highest (65.8%), followed by liquor puris (14.2%). The clinical samples were mainly taken in ICU, respiratory disease department and orthopedics department. No vancomycin resistant strains were detected. Totally 108 strains were methicillin resistant Staphylococcus aureus (MRSA). The positive rate of D-test was 65.5%. Conclusion S. aureus was mainly detected in respiratory samples, followed by liquor puris. The strains were resistant to commonly used antibiotics at different levels except vancomycin. The induced clindamycin resistance was serious. It is necessary to strengthen the surveillance and control of the drug resistance of S. aureus, and use D-test routinely to guide the rational use of antibiotics in clinical treatment.