Abstract: Objective To understand the category, epidemiological characteristics and drug resistance of pathogenic bacteria causing infectious diarrhea in Fengtai, Beijing, and provide evidence for the prevention and clinical treatment of bacterial diarrhea. Methods Stool samples were collected from diarrhea patients in 4 sentinel hospitals from 2010 to 2012 to conduct the detection of Salmonella, Shigella, Vibrio parahaemolyticus, Vibrio cholerae and Escherichia coli. The time, population and serotype distributions of the pathogens were analyzed by statistical methods. The susceptibility of 140 strains of pathogens to antibiotics was tested by Kirby-Bauer method recommended by US Clinical and Laboratory Standards Institute. Results Totally 357 strains of pathogens were isolated from 1108 specimens (32.22%). V. parahaemolyticus was predominant, accounting for 50.98%, followed by Salmonella (18.49%). The seasonality of the positive detection of pathogens was obvious, the detection rate was high during July-September. The differences in detection rate in different age groups were statistical significant (PV. parahaemolyticus between males and females was statistical significant (PV. parahaemolyticus was O3K6, the major serotype of Shigella was Shigella Sonnei and the major serotype of Salmonella were Salmonella Enteritidis and Salmonella senftenberg. The sensitivity of different pathogens to antibiotics varied. Most isolated strains were highly sensitive to cefoxitin, amoxicillin-clavulanic acid and aztreonam. Conclusion V. parahaemolyticus and Salmonella were the main pathogenic bacteria causing infectious diarrhea in Fengtai. The pathogen spectrum had changed. Different pathogenic bacterium had different resistance to antibiotics. The active surveillance of these pathogenic bacteria should be strengthened.