陈霞, 李桂喜, 禹惠兰, 袁敏, 侯小平, 李娟. 插入序列共同区在多种临床菌株间的分布及其与超广谱β-内酰胺酶基因blaPER-1的关系研究[J]. 疾病监测, 2013, 28(3): 178-183. DOI: 10.3784/j.issn.1003-9961.2013.3.004
引用本文: 陈霞, 李桂喜, 禹惠兰, 袁敏, 侯小平, 李娟. 插入序列共同区在多种临床菌株间的分布及其与超广谱β-内酰胺酶基因blaPER-1的关系研究[J]. 疾病监测, 2013, 28(3): 178-183. DOI: 10.3784/j.issn.1003-9961.2013.3.004
CHEN Xia, LI Gui-xi, YU Hui-lan, YUAN Min, HOU Xiao-ping, LI Juan. Distribution of IS CR1in clinical strains and its relationship with blaPER-1gene[J]. Disease Surveillance, 2013, 28(3): 178-183. DOI: 10.3784/j.issn.1003-9961.2013.3.004
Citation: CHEN Xia, LI Gui-xi, YU Hui-lan, YUAN Min, HOU Xiao-ping, LI Juan. Distribution of IS CR1in clinical strains and its relationship with blaPER-1gene[J]. Disease Surveillance, 2013, 28(3): 178-183. DOI: 10.3784/j.issn.1003-9961.2013.3.004

插入序列共同区在多种临床菌株间的分布及其与超广谱β-内酰胺酶基因blaPER-1的关系研究

Distribution of IS CR1in clinical strains and its relationship with blaPER-1gene

  • 摘要: 目的 研究临床来源的多种类细菌中插入序列共同区(insertion sequence common regions,ISCRs)及超广谱β-内酰胺酶基因blaPER-1的分布情况,分析可移动元件IS CR1 与细菌多药耐药表型及blaPER-1基因散播的关系。 方法 收集2011-2012年河北省某医院临床独立菌株,应用梅里埃VITEK® 2 COMPACT全自动微生物生化鉴定系统结合16S rDNA序列分析进行种属鉴定,利用普通聚合酶链反应(polymerase chain reaction,PCR)方法进行IS CR1、blaPER-1和IS CR1-blaPER-1间区的扩增;分析IS CR1、blaPER-1种属分布状况及IS CR1与菌株耐药性的关系。 结果 共得到17种(属)314株临床独立菌株,其中IS CR1携带率为28.7%(90/314),在不动杆菌和肠杆菌科的多种细菌中携带较多;携带IS CR1的菌株中,96.7%(87/90)为多药耐药菌株,93.3%(84/90)耐受9类及以上抗菌药物;blaPER-1基因携带率为12.4%(39/314),在7种细菌检出,不动杆菌属细菌中检出最多(31株);21株不动杆菌属细菌和1株普通变形杆菌存在IS CR1-blaPER-1结构。 结论 IS CR1在多种细菌中广泛分布,细菌多药耐药表型和其携带有一定关系;blaPER-1基因在多种细菌中散播,IS CR1的基因捕获和移动机制可能造成了blaPER-1基因在不动杆菌属细菌中及在不同种细菌间的散播。

     

    Abstract: Objective To understand the distribution of blaPER-1 gene and IS CR1in clinical isolates of different species and the relationships between IS CR1and multidrug resistance, and between IS CR1and blaPER-1 gene. Methods Clinical isolates were collected from a hospital in Hebei province during 2011-2012. The species of the isolates were identified by VITEK® 2 COMPACT automatic microbial identification system and 16S rDNA sequence analysis. The distributions of IS CR1, blaPER-1 gene and IS CR1-blaPER-1 were detected by PCR. Results A total of 314 clinical isolates of different species were collected. IS CR1and blaPER-1 gene were detected in 28.7% and 12.4% of the isolates, respectively. IS CR1-blaPER-1 sequence structure was detected in 1 Proteus vuigaris strain and 21 Acinetobacter spp. strains. Up to 96.7% of IS CR1contained strains were multidrug resistant, and 93.3% of them were resistant to all 9 antibiotics. Conclusion IS CR1was distributed in various species of clinical isolates widely, which was related with multidrug resistance in clinical bacteria. blaPER-1 gene was distributed in various species of clinical isolates. IS CR1might facilitate the spread of blaPER-1 gene among Acinetobacter spp. and other bacteria species.

     

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