梅玉发, 王喜云, 崔龙, 刘兰芳. 湖北省十堰市流感样病例标本采集对病毒分离效果影响因素分析[J]. 疾病监测, 2013, 28(4): 300-302. DOI: 10.3784/j.issn.1003-9961.2013.4.014
引用本文: 梅玉发, 王喜云, 崔龙, 刘兰芳. 湖北省十堰市流感样病例标本采集对病毒分离效果影响因素分析[J]. 疾病监测, 2013, 28(4): 300-302. DOI: 10.3784/j.issn.1003-9961.2013.4.014
MEI Yu-fa, WANG Xi-yun, CUI Long, LIU Lan-fang. Factors influencing virus isolation during sample collection from influenza-like illness cases in Shiyan, Hubei[J]. Disease Surveillance, 2013, 28(4): 300-302. DOI: 10.3784/j.issn.1003-9961.2013.4.014
Citation: MEI Yu-fa, WANG Xi-yun, CUI Long, LIU Lan-fang. Factors influencing virus isolation during sample collection from influenza-like illness cases in Shiyan, Hubei[J]. Disease Surveillance, 2013, 28(4): 300-302. DOI: 10.3784/j.issn.1003-9961.2013.4.014

湖北省十堰市流感样病例标本采集对病毒分离效果影响因素分析

Factors influencing virus isolation during sample collection from influenza-like illness cases in Shiyan, Hubei

  • 摘要: 目的 探讨流感样病例标本采集对病毒分离效果的影响因素,提高流感病原学监测质量。 方法 对2012年1-10月十堰市2家哨点医院送检的流感样病例标本进行质量分析。 结果 儿童监测点标本质量比成年人监测点标本质量高;发病后第1~2天采集的标本细胞分离阳性率最高,不同发病天数采集的标本阳性率差异有统计学意义(χ2=21.145, P=0.001);标本采集后于-20℃冰柜保存时间在4 d以内,阳性率均在60%以上,不会影响病毒的MDCK细胞分离。 结论 临床标本采集的质量及其保存运输等环节是病毒分离成功与否的主要影响因素。

     

    Abstract: Objective To understand the factors influencing the virus isolation during sample collection from influenza like illness(ILI) cases in Shiyan, Hubei province, and provide evidence for the improvement of influenza etiological surveillance. Methods The analysis was conducted on the quality of the clinical samples of ILI cases sent by 2 sentinel hospitals in Shiyan between January and October 2012. Results The quality of the samples collected from children was better than that from adults, the positive rate of the samples collected within 1-2 days after onset was highest, the difference in the positive rate of the samples collected at different days had statistical significance(χ2=21.145, P=0.001). The samples stored at-20℃ for less than 4 days had a positive rate of>60%. Conclusion The quality of clinical samples and store and transport conditions are the important factors related to the success of influenza virus isolation.

     

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