Abstract: Objective To understand the drug resistance of Mycobacterium tuberculosis in Quzhou and provide evidence for the control of tuberculosis (TB). Methods Mycobacterium tuberculosis strains were isolated from the samples of the TB patients receiving treatment in the hospitals in Quzhou from 2010 to 2012. The drug susceptibility of the isolated strains to 6 anti TB drugs isoniazid (INH), rifampicin (RFP), ethambutal (E); streptomycin (SM), levofloxacin (OFX) and kanamycin (KM) was tested. Results Among 1169 strains tested, the overall drug resistant rate was 22.80%, the overall multi drug resistant rate was 5.13% and the extensive drug resistant rate was 0.43%; The mono drug resistant pattern was INH.SM OFX, the poly drug resistant pattern was INH+SM, INH+SM+OFX, RFP+OFX and the multi drug resistant pattern was INH+RFP+SM, INH+RFP+SM+OFX and INH+RFP. Conclusion Drug resistance of Mycobacterium tuberculosis has posed great threat to the control of TB. It is important for the health department to take targeted measures to control the prevalence of drug-resistant TB.