Abstract: Objective To understand the current status of drug resistance of Acinetobacter baumannii and the resistance induced by genome blaoxa-23. Methods The susceptibility of 200 strains of A. baumannii to common antibiotics were tested by Kirby-Bauer method, and polymerase chain reaction was conducted to detect carbapenemases-related genome blaoxa-23 and the insertion sequence of ISAba1. Results The resistance rates of cefoperazone/sulbactam, imipenem and polymyxin were relatively low, but the resistant rates to other antibiotics were all above 80%. Among the 200 strains, 155 carried blaoxa-23 gene and 168 carried the insertion sequence of ISAba1. Conclusion The drug resistance of A. baumannii in our hospital was very serious, many A. baumannii strains carried blaoxa-23 gene, which would be responsible for their drug resistance, the insertion sequence of ISAba1 was often associated with the presence of blaoxa-23 gene.