Abstract: Objective To identify species composition of Candida clinical isolates and analyze the antibiotic resistance of the isolates. Methods VITEK Ⅱ, API20c, CHRO Magar and PCR method were used to identify yeast like clinical isolates, and the ATB Fungus3 was used for antibiotic resistance testing. Results A total of 96 yeast like clinical isolates from China Japan Friendship Hospital were identified, which belonged to 6 species, including 40 Candida albicans strains (41.7%), 36 Candida tropicalis strains (37.5%), 13 Candida glabrata strains (13.54%), 5 Candida parapsilosis strains (5.21%), 1 Candida krusei strain (1.04%) and 1 Candida Norway strain (1.04%). The strains showed different drug susceptibility to 5 anti fungal drugs tested. The strains were 100% sensitive to 5 flucytosine and amphotericin b, but showed different resistance to 3 azoles, including fluconazole, itraconazole and voriconazole. Conclusion Molecular analysis of the ITS region combined with traditional culturing is a effective way for the identification of Candida. In this study, the results indicated that infection of non albicans Candida was in increase. ICU is the place where Candida is frequently isolated. Patients aged >60 years are at high risk of the nosocomial infection. The results of anti fungi test in vitro showed that some Candida isolates had resistance to azole antifungal drugs.