Abstract: Objective To understand the genotypes of circulating Norovirus strains in Guangxi in 2012. Methods A total of 323 fecal samples collected from diarrheal children under 5 years old in 2012 were detected for Norovirus with RT-PCR, and corresponding sequences of partial VP1 region were analyzed and phylogenetic tree was constructed. Results Among the samples detected, twenty-four were Norovirus positive. Based on partial sequences of VP1 region, two strains belonged to genotype Ⅰ, twenty-two belonged to genotype Ⅱ. Three of 22 GⅡ strains collected in November and December belonged to GⅡ.4 Sydney 2012 cluster,eighteen strains belonged to the 2006b cluster and one belonged to the GⅡ.6 cluster. Conclusion The circulating Norovirus genotypes were with diversity, and Norovirus GⅡ.4 Sydney variant was first detected in Guangxi.