王森, 王永全, 苗元, 高志勇, 崔京辉, 胡晓芬, 靳博. 2013年北京地区5岁以下儿童病毒性腹泻病原学研究[J]. 疾病监测, 2014, 29(5): 344-348. DOI: 10.3784/j.issn.1003-9961.2014.05.004
引用本文: 王森, 王永全, 苗元, 高志勇, 崔京辉, 胡晓芬, 靳博. 2013年北京地区5岁以下儿童病毒性腹泻病原学研究[J]. 疾病监测, 2014, 29(5): 344-348. DOI: 10.3784/j.issn.1003-9961.2014.05.004
WANG Sen, WANG Yong-quan, MIAO Yuan, GAO Zhi-yong, CUI Jing-hui, HU Xiao-fen, JIN Bo. Etiology of viral diarrhea in children aged5 years in Beijing, 2013[J]. Disease Surveillance, 2014, 29(5): 344-348. DOI: 10.3784/j.issn.1003-9961.2014.05.004
Citation: WANG Sen, WANG Yong-quan, MIAO Yuan, GAO Zhi-yong, CUI Jing-hui, HU Xiao-fen, JIN Bo. Etiology of viral diarrhea in children aged5 years in Beijing, 2013[J]. Disease Surveillance, 2014, 29(5): 344-348. DOI: 10.3784/j.issn.1003-9961.2014.05.004

2013年北京地区5岁以下儿童病毒性腹泻病原学研究

Etiology of viral diarrhea in children aged5 years in Beijing, 2013

  • 摘要: 目的 了解北京地区5岁以下儿童病毒性腹泻的流行病学特征,为病毒性腹泻的防治提供理论依据。 方法 全年随机采集北京市2家三级甲等医院的5 岁以下腹泻患儿粪便标本,利用酶联免疫吸附试验检测轮状病毒(HRV)、用聚合酶链反应(PCR)检测杯状病毒(HuCV)、肠道腺病毒(AdV)及星状病毒(AstV)。 结果 研究期间共收集病毒性腹泻患儿粪便标本243份,标本总阳性检出率52.7%(128/243),4种病毒阳性检出率分别为HRV(26.3%,64/243)、HuCV(16.9%,41/243)、AdV(4.9%,12/243)和AstV(4.5%,11/243),分别占总阳性检出的49.9%、32.1%、9.3%和8.5%(其中包括混合感染10例,占总阳性检出的7.8%)。患者以2岁儿童为主,不同年龄组之间病毒总检出率差异有统计学意义(2 =13.397,P=0.009),不同病毒发病高峰期不同,男女罹患率差异无统计学意义(2=0.220,P0.05)。 结论 HRV和HuCV是引起婴幼儿病毒性腹泻的主要病原体;4种病毒总检出率以12~18月龄组最高;全年中以12月的病毒检出率最高;存在一定比例的混合感染。

     

    Abstract: Objective To understand the etiological characteristics of viral diarrhea in children aged5 years in Beijing and provide evidence for the prevention and treatment of viral diarrhea. Methods Stool samples were collected randomly from diarrheal children aged5 years in 2 grade A hospitals in Beijing in 2013. Enzyme-linked immunosorbent assay (ELISA) was conducted to detect rotavirus. Polymerase chain reaction (PCR) was conducted to detect calicivirus, enteric adenovirus and astrovirus. Results A total of 243 stool samples were collected. Among these samples,the overall positive rate in virus detection was 52.7% (128/243). The positive rate was 26.3% (64/243) for rotavirus, 16.9% (41/243) for calicivirus, 4.9% (12/243) for enteric adenovirus and 4.5% (11/243) for astrovirus. The positive samples of 4 pathogens accounted for 49.9%, 32.1%, 9.3% and 8.5% of total positive samples (including 10 co-infections, accounting for 7.8% of total positive samples).Most cases were children aged2 years. The differences in virus detection rate among different age groups had statistical significance (2 =13.397, P=0.009). The incidence peal varied with virus. The gender specific difference in attack rate had no statistical significance (2=0.220, P0.05). Conclusion Rotavirus and calicivirus were the major pathogens causing viral diarrhea in children. The detection rate of 4 pathogens was highest in age group 12-18 months. The seasonal detection rate was highest in December. Co-infections were detected too.

     

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