张建群, 李永东, 罗学辉, 袁士杰. 2010-2012年浙江省余姚市手足口病的病原构成及CoxA16VP1基因特征分析[J]. 疾病监测, 2014, 29(11): 867-870. DOI: 10.3784/j.issn.1003-9961.2014.11.007
引用本文: 张建群, 李永东, 罗学辉, 袁士杰. 2010-2012年浙江省余姚市手足口病的病原构成及CoxA16VP1基因特征分析[J]. 疾病监测, 2014, 29(11): 867-870. DOI: 10.3784/j.issn.1003-9961.2014.11.007
ZHANG Jian-qun, LI Yong-dong, LUO Xue-hui, YUAN Shi-jie. Constituent of pathogens causing hand foot and mouth disease and genetic characteristics of Coxsackie virus A 16 in Yuyao, 2010-2012[J]. Disease Surveillance, 2014, 29(11): 867-870. DOI: 10.3784/j.issn.1003-9961.2014.11.007
Citation: ZHANG Jian-qun, LI Yong-dong, LUO Xue-hui, YUAN Shi-jie. Constituent of pathogens causing hand foot and mouth disease and genetic characteristics of Coxsackie virus A 16 in Yuyao, 2010-2012[J]. Disease Surveillance, 2014, 29(11): 867-870. DOI: 10.3784/j.issn.1003-9961.2014.11.007

2010-2012年浙江省余姚市手足口病的病原构成及CoxA16VP1基因特征分析

Constituent of pathogens causing hand foot and mouth disease and genetic characteristics of Coxsackie virus A 16 in Yuyao, 2010-2012

  • 摘要: 目的 了解浙江省余姚市引起手足口病的病原构成,研究Cox A16基因特征及变异情况.方法 收集手足口病患者标本,用实时荧光定量聚合酶链反应(real-time PCR)检测并分型,每年选择几个样品进行Cox A16 VP1基因的扩增和核苷酸序列测定,测序结果利用DNAstar和Blast软件进行比对分析.结果 2010-2012年从1651份标本检出柯萨奇病毒A组16型(Cox A16)25.86%,肠道病毒71型(EV71) 39.85%,其他肠道病毒19.38%; Cox A16 VP1蛋白氨基酸序列相似性为99.3%~100%,7株Cox A16基因分型属于B1基因亚型.结论 2010-2012年余姚市手足口病流行的主要病原体为Cox A16 和EV71,流行的Cox A16属于B1基因亚型,并具有高度相似性.

     

    Abstract: Objective To understand the constituent of pathogens causing hand foot and mouth disease (HFMD) and the genetic characteristics of Coxsackie virus A 16 (Cox A16). Methods The stool samples collected from HFMD patients were detected with real time fluorescence polymerase chain reaction. Several stool samples were selected every year to conduct the VP1 gene amplification and nucleotide sequencing of Cox A16, and the sequencing results were compared and analyzed with software DNAstar and Blast. Results From 2010 to 2012, a total of 1651 stool samples were detected, in which 25.86% were Cox A16 positive, 39.85% were EV71 positive and 19.38% were other enterovirus positive. The VP1 protein amino acid sequence of the Cox A16 strains detected shared 99.3% to 100% similarity. The 7 Cox A16 strains belonged to genotype B1. Conclusion Cox A16 and EV71 were the predominant pathogens causing HFMD in Yuyao during 2010-2012, and the circulating Cox A16 belonged to genotype B1 with high similarity.

     

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