Abstract: Objective To analyze the immune effect of bivalent vaccine of hemorrhagic fever with renal syndrome (HFRS) in Xianyang, Shaanxi province, and provide scientific evidence for the prevention strategy based on this vaccine. Methods A total of 100 subjects were selected to compare their antibody level before the vaccination, after basic vaccination, after booster vaccination and 1 year after booster vaccination. Results After basic vaccination, the overall antibody level to hantaan virus significantly increased, and reached its peak after booster vaccination, then begin to decline. Correlations between the antibody level and the area and age were not obvious. The antibody positive rate was 4.26% before vaccination and 41.49% after basic vaccination. The antibody positive rate was 61.70% after booster vaccination and 47.87% 1 year after booster vaccination. By McNemar test, the antibody seroconversion rate after basic vaccination was 38.89% (35/90) and the antibody seroconversion rate reached 61.11% after booster vaccination (55/90). Conclusion The antibody level and antibody-positive rate to hantaan virus increased obviously after bivalent HFRS vaccine immunization, indicating that the vaccine immunization is a fast, safe and effective measure for HFRS prevention and control.